Antioxidant Activity Of Medicinal Plants Thesis

Standards and different extracts were prepared at 1 mg/ml and 5 mg/ml in HPLC grade methanol, respectively. Characterization of the peaks was made according to their amplitude.Identification was performed using retention times and available standards.Copyright © 2019 Sabine Adeline Fanta Yadang et al.

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Five hundred grams (500g) of powder were macerated, respectively, with 3 L of ethanol (70% ethanol-30% water), methanol, and water for 2 days.

The macerate obtained was filtered and evaporated using a rotary evaporator (BUCHI Rotavapor R-300) at 50°C.

Therefore, an antioxidant intake is needed to reduce the excess of the ROS and thus suppress oxidative damage.

An antioxidant is a substance that even at low concentration delays and prevents the oxidation of the substrate [3].

Under normal conditions, the level of free radicals is controlled by an antioxidant defense system that decreases oxidative damage [1].

Oxidative stress is considered a major contributor to many chronic diseases [2].

Carissa edulis is a plant used in the management of oxidative stress and inflammatory related disorders such as malaria, rheumatism inflammation, and cardiovascular diseases.

The present study evaluates the total phenolic content, antioxidant capacity (DPPH, ABTS, and FRAP), and the bioactive compounds present in the various extracts of C. An HPLC analysis determined the different compounds present in the extracts.

Mitochondrial oxidative phosphorylation, which is a process that leads to energy production, is a phenomenon that occurs in most aerobic organisms.

The partial electron transfer of oxygen molecules during this metabolism leads to the production of unstable molecules such as the superoxide anion O which is a nonradical species but is capable of liberating hydroxyl radicals according to the Fenton reaction.

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