As shown in Table 1, addition of sodium bicarbonate to the nitrate and phosphate deficit cultures (NThe aim of the study was to investigate the effect of sodium bicarbonate on carbohydrate and lipid content of D. Among all macronutrient stresses, nitrogen stress amended with bicarbonate was found to be the ideal method for production of algal lipids, as it resulted in comparatively lower biomass with high carbohydrate and lipid contents in D. Effect of bicarbonate on protein, carbohydrate and lipid content of dry cell weight (DCW) of D. P cultures with or without bicarbonate did not have any significant effect on the protein content of D.
salina compared to the standard control with adequate nutrients.
salina, which indicates the active utilization of bicarbonate for the growth and metabolic process of microalgae under stress conditions.
The effect of bicarbonate on photosynthesis efficiency (Fv/F) of Dunaliella cultures under nutrient deficit conditions were presented in Fig. Maximum photosynthesis efficiency was observed in the nutrient deficit Dunaliella cultures amended with bicarbonate, whereas all nutrient deficit cultures (N) of Dunaliella cultures during nutrient deficit conditions.
ROS is known to be an important factor in cellular response and its detrimental role at higher concentrations has been well-established in photosynthetic microalgae under nutrient stress.
To our knowledge, this is the first report studying the effect of bicarbonate amendment on antioxidant response of D. Our study focuses on evaluating the combined effect of sodium bicarbonate and macronutrient starvation such as nitrate (N with or without amendment of bicarbonate were studied for 28 days (Fig. The Dunaliella cells were subjected to these three different nutrient stresses in the presence and absence of sodium bicarbonate (100 m M).
Carbon dioxide can be taken up and utilized by microalgae in two forms, namely inorganic carbon (sodium bicarbonate) and carbon-dioxide gas.
Only a few algae have the ability to directly take up gaseous CO.
Thus, we determined the relative content of FAMEs in the experimental conditions, using GC-MS (Table S1 of Supplementary Material).
Maximum increase in fatty acid accumulation was achieved in N (17.31 ± 0.09 and 18.9 ± 1.67), which was significantly (two folds) higher that of control culture.